辐照对带鱼鱼糜内源性转谷氨酰胺酶及其凝胶特性的影响

摘要/Abstract

摘要： 内源性转谷氨酰胺酶（transglutaminase, TGase）与鱼糜凝胶性能密切相关，辐照处理会引起鱼糜TGase结构与活性的变化。为考察电子束辐照对带鱼鱼糜品质的影响，采用不同剂量电子束处理带鱼鱼糜，测定鱼糜内源性TGase的酶活及其最适温度、最适pH，傅里叶红外光谱和圆二色光谱分析TGase构象单元，结合鱼糜凝胶强度和持水性的变化，探究电子束辐照对鱼糜内源性TGase及其凝胶性能的影响机理。结果显示，随着电子束辐照剂量的增加，鱼糜内源性TGase活性呈先上升后下降，5 kGy剂量辐照能显著提高鱼糜TGase酶活，鱼糜凝胶强度及其保水性达到最大值；各组TGase的最适pH均为8.0，除9 kGy组外，其余剂量辐照组和对照组TGase的最适温度均为40℃；辐照引起鱼糜TGase分子中的二级结构单元转变，5 kGy组TGase的α-螺旋和β-转角含量达最小值，而β-折叠及无规卷曲含量达最大值。结论：电子束辐照影响带鱼鱼糜内源性TGase二级结构及其酶活，适宜剂量促进TGase分子中α-螺旋和β-转角结构转化为β-折叠及无规卷曲，有利于提高鱼糜TGase活性，促进带鱼鱼糜凝胶的形成。

关键词: 电子束辐照, 内源性转谷氨酰胺酶, 二级结构, 带鱼鱼糜, 凝胶强度

Abstract: Endogenous transglutaminase (TGase) is closely related to the properties of surimi gel, and irradiation treatment may cause the changes in the structure and activity of TGase. To investigate the effect of electron beam (EB) irradiation with different doses on the quality of the hairtail (Trichiurus haumela) surimi, the enzyme activity, optimum temperature and pH of TGase were determined, and the conformation unit of TGase was analyzed with Fourier transform infrared spectroscopy and circular dichroism. Combinded with the changes of gel strength and water holding capacity in surimi gel, the mechanism of EB irradiation on the endogenous TGase and the gel properties of surimi were investigated. Results showed that the activity of TGase increased first and then decreased with the increase of EB irradiation dose. The irradiation of 5 kGy dose significantly increased the activity of TGase, and the highest gel strength and water holding capacity were observed. The optimum pH of TGase in each group was 8.0, and the optimum temperature of TGase both in irradiation group and control group were 40℃, except for the 9 kGy group. Furthermore, EB irradiation caused the transform of secondary structural units in TGase molecule, the lowest content of α-helix and β-turn, and highest content of β-sheet and random coil were observed at 5 kGy, respectively. It was concluded that EB irradiation affected the secondary structure and enzyme activity of TGase in hairtail surimi. In addition, the appropriate dose could promote the transformation of α-helix and β-turn into β-sheet and random coil, and improve the activity of TGase, which promoted the gelation of hairtail surimi.

Key words: electron beam irradiation, endogenous transglutaminase, secondary structure, hairtail surimi, gel strength

中图分类号:

TS254.1

杨文鸽. 辐照对带鱼鱼糜内源性转谷氨酰胺酶及其凝胶特性的影响[J]. 食品科学, 0, (): 0-0.

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