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芳香中药纯露对鲜切双孢菇的抗氧化及保鲜作用

吴克刚1,黄煜强2,余冰莹2,3,崔正祥2,段雪娟2,柴向华2,张志辉3,4,郑鹏飞3,4   

  1. 1. 广东工业大学轻工化工学院
    2. 广东工业大学
    3.
    4. 英德原野阳光生物科技发展有限公司
  • 收稿日期:2022-09-27 修回日期:2023-06-04 出版日期:2023-08-15 发布日期:2023-08-29
  • 通讯作者: 黄煜强 E-mail:hyq172124@163.com

Antioxidant and Anti-enzymatic Browning Effect of Aromatic Hydrosol and its Application in Fresh-cut Mushroom Preservation

2,2, 2, 2, 2,2, 2,2   

  • Received:2022-09-27 Revised:2023-06-04 Online:2023-08-15 Published:2023-08-29

摘要: 在鲜切果蔬产品产生的褐变中,多酚氧化酶(Polyphenol Oxidase, PPO)是最重要的因素之一,本文研究芳香中药纯露对PPO活性的影响以及其生物抗氧化作用。水蒸气蒸馏法提取的12种芳香纯露体外对PPO活性均有抑制作用,其中8种芳香纯露对DPPH、ABTS和PTIO自由基有较强的清除作用,总抗氧化能力实验表明芳香纯露具有较强的抗氧化活性。采用电子鼻和气质联用(GC-MS)分析芳香纯露中的挥发性成分,萜类化合物和芳香族化合物为主要组分。将提取的芳香纯露应用于保鲜双孢菇的并测试相关理化性质,结果显示,与对照组相比,芳香纯露处理的双孢菇体内PPO活性被抑制,褐变指数(Browning Index, BI)延缓增长。此外,总酚含量、硬度、重量损失和微生物菌落总数都得到有效控制。这些结果表明,使用芳香纯露处理对于保持鲜切双孢菇的品质有积极作用。

关键词: 芳香中药, 纯露, PPO, 抗氧化, 双孢菇保鲜

Abstract: Polyphenol Oxidase (PPO) is one of the most important factors affecting the browning of fresh-cut fruit and vegetable products. In this paper, the effect of aromatic hydrosol on the activity of PPO and its biological antioxidant effect were studied. Twelve kinds of aromatic plants were extracted by steam distillation, and they all inhibited the activity of PPO. Among them, 8 kinds of hydrosol had a scavenging effect on free radicals of DPPH, ABTS, and PTIO. The experiment on total antioxidant capacity showed that they had strong antioxidant activity. The volatile components in aromatic hydrosol were analyzed by electronic nose and gas chromatography-mass spectrometry (GC-MS). The result shows that terpenoids and aromatic compounds were the main components. Aromatic hydrosol was used to fresh-keeping mushrooms and the related physical and chemical properties were tested. The results showed that compared with the control group, the PPO activity in the mushrooms treated with aromatic hydrosol was inhibited, and the Browning Index (BI) was delayed. In addition, total phenol content, hardness, weight loss and total microbial colonies are effectively controlled. These results indicate that the use of aromatic hydrosol treatment has a positive effect on maintaining the quality of fresh-cut mushrooms.

Key words: Aromatic Hydrosol, Polyphenol Oxidase, (PPO), Antioxidation, Mushroom Preservation