关键词: 葡萄糖氧化酶, 酶活力, 培养基优化, 响应面

Abstract:

Glucose oxidase (GOD) has gained considerable commercial importance due to its multitude of applications in the food, pharmaceutical, biotechnology and other industries. In the present investigation, a high GOC-producing strain was isolated from the soil of Wenwu Xuefeng farm in Fuzhou, and the fermentation medium was optimized by response surface methodology for higher GOD production by the isolated strain. The optimal medium composition determined consisted of glucose 109.41 g/L, complex nitrogen source (proteose peptone + (NH₄)₂SO₄, 3:1, m/m) 37.36 g/L, Tween-80 37.15 g/L, MgSO₄·7H₂O 0.7 g/L, KH₂PO₄ 2 g/L and KCl 0.5 g/L. A maximum GOD enzyme activity of 1.54 U/mL was obtained using the optimized fermentation medium, 31.6% higher than before optimization.

Key words: glucose oxidase, enzyme activity, medium optimization, response surface methodology