一水肌酸及共轭亚油酸对大鼠肌纤维相关基因和酶活性的影响及机理

doi:10.7506/spkx1002-6630-20200915-183

食品科学 ›› 2021, Vol. 42 ›› Issue (7): 149-155.doi: 10.7506/spkx1002-6630-20200915-183

一水肌酸及共轭亚油酸对大鼠肌纤维相关基因和酶活性的影响及机理

苏琳，李慧姣，侯艳茹，赵雅娟，白艳苹，孙冰，赵丽华，靳烨

（内蒙古农业大学食品科学与工程学院，内蒙古呼和浩特 010018）

出版日期:2021-04-15 发布日期:2021-05-17
基金资助:
国家自然科学基金地区科学基金项目（32060519）；内蒙古自治区自然科学基金重大专项（2020ZD11）；内蒙古自治区科技计划项目（201802067）

Effects of Dietary Supplementation with Creatine Monohydrate and Conjugated Linoleic Acid on Muscle Fiber-Related Gene Expression and Enzyme Activities in Mice

SU Lin, LI Huijiao, HOU Yanru, ZHAO Yajuan, BAI Yanping, SUN Bing, ZHAO Lihua, JIN Ye

(College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China)

Online:2021-04-15 Published:2021-05-17

摘要/Abstract

摘要： 本实验旨在研究饲粮中添加一水肌酸（creatine monohydrate，CMH）和共轭亚油酸（conjugated linoleic acid，CLA）对大鼠肌纤维相关基因及酶活性的影响及机理。选择40 只健康雌性Wistar大鼠随机分为5 组：对照组，饲喂基础饲料；CMH低剂量组，饲喂0.5%（以基础饲料质量计，下同）CMH＋基础饲料；CMH高剂量组，饲喂1% CMH＋基础饲料；CLA低剂量组，饲喂0.5% CLA＋基础饲料；CLA高剂量组，饲喂1% CLA＋基础饲料；饲喂4 周。结果表明：与对照组相比，CMH低剂量组大鼠日摄入量显著降低（P＜0.05），肌球蛋白重链IIa（myosin heavy chain IIa，MyHC IIa）、MyHC IIb、过氧化物酶增殖体激活受体γ辅激活因子1α（peroxisome proliferator-activated receptor γ coactivator 1α，PGC-1α）、肌细胞增强因子2C（myocyte enhancer factor 2C，MEF2C）、葡萄糖转运子4（glucose transporter 4，GLUT4）mRNA相对表达量及琥珀酸脱氢酶（succinate dehydrogenase，SDH）活力显著增加（P＜0.05）；CMH高剂量组大鼠日摄入量显著降低（P＜0.05），MyHC IIb、MEF2C、GLUT4 mRNA相对表达量显著增加（P＜0.05）。与对照组相比，CLA低剂量组大鼠日摄入量、MyHC IIx mRNA相对表达量显著降低（P＜0.05），MyHC IIa、PGC-1α mRNA相对表达量显著升高，SDH、苹果酸脱氢酶、一磷酸腺苷活化蛋白激酶（AMP-activated protein kinase，AMPK）活力显著增加（P＜0.05）；CLA高剂量组大鼠日摄入量、乳酸脱氢酶活力、MEF2C mRNA相对表达量显著降低（P＜0.05），MyHC I、MyHC IIa、PGC-1α、GLUT4 mRNA相对表达量和SDH活力显著增加（P＜0.05）。综上所述，饲粮添加CMH、CLA后，大鼠肌肉组织中AMPK被激活，氧化代谢酶活性增加，通路相关基因PGC-1α、MEF2C、GLUT4 mRNA相对表达量增加，肌肉氧化代谢增强，进而改变肌纤维类型。

关键词: 一水肌酸；共轭亚油酸；肌纤维类型；酶活力；肌纤维调控基因

Abstract: The aim of this study was to investigate the effects and underlying mechanisms of dietary supplementation with creatine monohydrate (CMH) and conjugated linoleic acid (CLA) on muscle fiber-related gene expression and enzyme activities in rats. Totally 40 healthy female Wistar rats were randomly divided into five groups coded A to E. Group A was fed a basic diet as the control group, group B the basic diet supplemented with 0.5% CMH, group C the basic diet supplemented with 1% CMH, group D the basic diet supplemented with 0.5% CLA , and group E the basic diet supplemented with 1% CLA. The feeding period lasted for four weeks. Compared to group A, group B showed a significant decrease in daily feed intake (P < 0.05), and a significant increase in the mRNA expression of myosin heavy chain IIa (MyHC IIa) and MyHC IIb, peroxisome-activated receptor coactivator 1α (PGC-1α), myocyte enhancer factor 2C (MEF2C), glucose transporter 4 (GLUT4), and succinate dehydrogenase (SDH) activity (P < 0.05); group C presented a significant decrease in daily feed intake (P < 0.05), and a significant increase in the mRNA expression of MyHC IIb, MEF2C and GLUT4 (P < 0.05). Compared to group A, group D showed a significant decrease daily feed intake and the mRNA expression of MyHC IIx (P < 0.05), and a significant increase in the mRNA expression of MyHC IIa and PGC-1α, and the activity of SDH, malate dehydrogenase, adenosine 5’-monophosphate-activated protein kinase (AMPK) (P < 0.05); group E exhibited a significant decrease in daily feed intake, lactic dehydrogenase (LDH) activity and the mRNA expression of MEF2C (P < 0.05), and a significant increase in the mRNA expression of MyHC I, MyHC IIa, PGC-1α and GLUT4, as well as SDH activity (P < 0.05). In summary, dietary supplementation with CMH or CLA activated AMPK in the muscle tissue of rats and increased the oxidative metabolism-related enzyme activities, up-regulated the mRNA expression of the muscle fiber-related genes PGC-1α, MEF2C and GLUT4, and enhanced the energy required for oxidative metabolism in muscle, thereby altering muscle fiber types.

Key words: creatine monohydrate; conjugated linoleic acid; muscle fiber type; enzymatic activity; muscle fiber type-related genes

中图分类号:

TS251.1

苏琳，李慧姣，侯艳茹，赵雅娟，白艳苹，孙冰，赵丽华，靳烨. 一水肌酸及共轭亚油酸对大鼠肌纤维相关基因和酶活性的影响及机理[J]. 食品科学, 2021, 42(7): 149-155.

SU Lin, LI Huijiao, HOU Yanru, ZHAO Yajuan, BAI Yanping, SUN Bing, ZHAO Lihua, JIN Ye. Effects of Dietary Supplementation with Creatine Monohydrate and Conjugated Linoleic Acid on Muscle Fiber-Related Gene Expression and Enzyme Activities in Mice[J]. FOOD SCIENCE, 2021, 42(7): 149-155.

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一水肌酸及共轭亚油酸对大鼠肌纤维相关基因和酶活性的影响及机理

Effects of Dietary Supplementation with Creatine Monohydrate and Conjugated Linoleic Acid on Muscle Fiber-Related Gene Expression and Enzyme Activities in Mice

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