关键词: 血红蛋白, 铬, 螯合物, 响应面分析

Abstract:

In order to optimize the preparation processing of chromium amino acid chelated, response surface methodology was employed to analyze the effects of reaction temperature, reaction time, pH, protein hydrolysate-chromium ratio and the cross-interaction among factors on chelating reaction. On the basis of single-factor experimental results, a mathematical regression model was established, and the optimal preparation processing conditions were reaction temperature of 48.9 ℃, reaction time of 85.57 min, pH 7.15, protein hydrolysate-chromium ratio of 3.76:1. Under these optimal processing conditions, the chelating rate was 0.6013, which was close to theoretical chelating rate of 0.6135 with a relative error of 1.99%. Therefore, this investigation provided a theoretical reference for the chromium supplementary products.

Key words: haemoglobin, chromium, chelate, response surface analysis