八氢番茄红素脱氢酶(PDS)基因果实特异表达载体的构建

doi:10.7506/spkx1002-6630-201013045

食品科学 ›› 2010, Vol. 31 ›› Issue (13): 196-199.doi: 10.7506/spkx1002-6630-201013045

八氢番茄红素脱氢酶(PDS)基因果实特异表达载体的构建

刘顺枝，孙莉丽，杨礼香，王小兰*

广州大学生命科学学院

收稿日期:2009-10-19 修回日期:2010-04-16 出版日期:2010-07-01 发布日期:2010-12-29
通讯作者: 王小兰 E-mail:wxl1972@gzhu.edu.cn
基金资助:
广州市科技计划项目(2008J1-C251-2)；广州市属高校科技计划项目(08C030)

Construction of Phytoene Desaturase (Pds) Gene Expression Vector with Tomato Fruit Specific E8 Promoter

LIU Shun-zhi，SUN Li-li，YANG Li-xiang，WANG Xiao-lan *

School of Biological Sciences, Guangzhou University, Guangzhou 510006, China

Received:2009-10-19 Revised:2010-04-16 Online:2010-07-01 Published:2010-12-29
Contact: WANG Xiao-lan E-mail:wxl1972@gzhu.edu.cn

摘要/Abstract

摘要：

八氢番茄红素脱氢酶(Pds)是番茄红素合成的关键酶，本研究通过PCR 法获取pds 基因和E8 启动子序列，将目的基因和E8 启动子序列构建到植物表达载体pMD1 中，构建了含果实特异表达启动子的pds 基因的植物表达载体。并采用PCR、限制性内切酶酶切和序列测定分析法，对重组质粒进行鉴定。结果表明：番茄果实特异性表达pds 的重组质粒构建成功；通过农杆菌直接转化技术将其成功转入转化农杆菌LBA4404、EHA105 中，为下一步pds 在番茄果实中特异表达奠定了基础。

关键词: 八氢番茄红素脱氢酶, 果实特异性启动子, 载体构建

Abstract:

Phytoene desaturase (Pds) is a key enzyme in the biosynthesis pathway of carotenoid. The target gene pds and the sequence of tomato fruit specific E8 promoter were amplified by PCR and then cloned into pMD1 vector, respectively. PCR, restriction enzyme map, and sequence analysis were used to identify the reconstructed plasmid. The experimental results confirmed the successful construction of a recombinant plasmid carrying pds gene and promoter E8. The recombinant plasmid successfully transformed Atumefaciens LBA4404 and EHA105. These investigations will provide references for further study of pds expression in tomato fruits.

Key words: phytoene desaturase, fruit specific promoter, vector construction

中图分类号:

Q966
Q786

刘顺枝，孙莉丽，杨礼香，王小兰*. 八氢番茄红素脱氢酶(PDS)基因果实特异表达载体的构建[J]. 食品科学, 2010, 31(13): 196-199.

LIU Shun-zhi，SUN Li-li，YANG Li-xiang，WANG Xiao-lan *. Construction of Phytoene Desaturase (Pds) Gene Expression Vector with Tomato Fruit Specific E8 Promoter[J]. FOOD SCIENCE, 2010, 31(13): 196-199.

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八氢番茄红素脱氢酶(PDS)基因果实特异表达载体的构建

Construction of Phytoene Desaturase (Pds) Gene Expression Vector with Tomato Fruit Specific E8 Promoter

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