食品科学 ›› 2010, Vol. 31 ›› Issue (9): 177-181.doi: 10.7506/spkx1002-6630-201009040

• 生物工程 • 上一篇    下一篇

土壤中正己醇降解菌的多样性研究

张锦华,师俊玲*   

  1. 西北农林科技大学食品科学与工程学院
  • 收稿日期:2009-08-26 修回日期:2010-02-20 出版日期:2010-05-01 发布日期:2010-12-29
  • 通讯作者: 师俊玲 E-mail:sjlshi2004@yahoo.com.cn
  • 基金资助:

    国家葡萄产业技术体系项目(nycytx-30-ch-03);西北农林科技大学科技创新专项(cx200905)

Diversity of Hexanol-degrading Strains in Soil

ZHANG Jin-hua,SHI Jun-ling*   

  1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
  • Received:2009-08-26 Revised:2010-02-20 Online:2010-05-01 Published:2010-12-29
  • Contact: SHI Jun-ling E-mail:sjlshi2004@yahoo.com.cn

摘要:

目的:筛选能够有效降低正己醇含量的微生物菌种,了解自然界中正己醇降解菌的分布与多样性。方法:以正己醇生产厂废水排放口周围的土壤为分离源,通过富集培养和分离筛选。结果:获得了6 株对正己醇有显著降解作用的菌种,根据其在PDA 培养基上的菌落形态和孢子形态初步分类鉴定为镰孢菌属、地霉属、毛霉属和曲霉属。其中,地霉属的菌株最多(3 株),对正己醇的降解能力强,适用pH 值范围广。地霉属菌株S12 和S13在中性和酸性条件下对3g/L 正己醇的降解率均达60% 以上。结论:自然界中的正己醇降解菌在种类、生态、降解能力、作用条件等方面都具有丰富的多样性。

关键词: 正己醇, 多样性, 正己醇降解菌, 分离筛选, 分类鉴定

Abstract:

Hexanol is an unfavorable flavor in food. Too much hexanol in food is harmful for human health. Screening and understanding diversity of hexanol-degrading microorganisms are helpful for biological degradation of hexanol and its potential application in food industry, which will provide basic information for further research on hexanol-degrading mechanisms. Soil around wastewater outfalls of hexanol-producing plants was used as the resources to screen hexanoldegrading microorganisms. After enrichment and isolation, 6 strains with degradation capability for hexanol were obtained. Based on morphological characteristics of colonies and spores, 6 strains were classified as fungi, which belonged to Fusarium Link, Geotrichum Link, Mucor Link and Aspergillus P. Micheliex Link, respectively. Geotrichum Link had 3 isolated strains, which exhibited higher hexanol-degrading capacity and wider suitability of pH. The strains in Geotrichum Link, S12 and S13 could result in the degradation of 3 g/L hexanol up to 60% under both neutral and acidic conditions. The rich diversity of hexanol-degrading strains in soil was reflected in species, ecology, degradation capacity, conditions and other aspects.

Key words: hexanol, diversity, hexanol-degrading strains, isolation, screening, identification

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