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Determination of Flavonoids from Potentilla freyninan by HPLC-MS/MS

LI Rong,ZHOU Yuan,YANG Li-chen,JIANG Zi-tao   

  1. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science,Tianjin University of Commerce, Tianjin 300134, China
  • Online:2013-07-25 Published:2013-08-02

Abstract:

Total flavonoids were obtained from Potentilla freyninan by microwave-assisted extraction method using 75%
ethanol as the extraction solvent and purified with D101 macroporous resin. The purified total flavonoids were analyzed
by high performance liquid chromatography tandem quadrupole mass spectrometry (HPLC-MS/MS) in the negative ion
monitoring mode. Based on relative molecular weight, MS2 fragmentation pattern, and retention time in liquid chromatography,
eight flavonoids in P. freyninan were identified, including 2,3,5,4’-tetrahydroxystilbane-2-O-β-D-glucoside, quercetin-3,7-
rutinosogalactoside, apigenin-8-C-glucoside, rutin, hyperoside, hesperidin, quercitrin, and kaempferol-3-rutinoside.

Key words: Potentilla freyninan, HPLC-MS/MS, flavonoids, hesperidin, hyperoside