Abstract:

In order to remove salt from grass carp protein hydrolysate, four macroporous adsorption resins including
DA201-C, DA201-M, SQT-67 and D002 were selected for the highest adsorption capacity for grass carp peptides through
static adsorption and desorption experiments. The results showed that DA201-C had the best adsorption efficiency for
grass carp peptides. Its adsorption quantity increased with increasing concentration of grass carp peptides. Seventy-five
percent ethanol revealed the best elution efficiency for DA201-C. The optimal conditions for the desalination of grass carp
peptides with DA201-C were determined as follows: the sample at 30 mg/mL was passed through the column at a flow rate
0.5 BV/h, and then the column was washed with at a flow rate of 2 BV/h and eluted with 75% ethanol at 2 BV/h. Under
these conditions, the desalination efficiency of grass carp peptides was 97.17%.

Key words: grass carp, protein hydrolysate, macroporous adsorption resin, adsorption, desorption