Isolation and Identification of Two Soil Actinomycetes ZSM-1 and ZSM-2

doi:10.7506/spkx1002-6630-201611020

Abstract

Abstract:

Actinomycete strains ZSM-1 and ZSM-2 isolated from soil were studied for their taxonomy. The 16S rDNA was
amplified by polymerase chain reaction (PCR) after the extraction of mycelial genomic DNA and the amplification products
were subjected to sequencing and homology analysis. A phylogenetic tree was established based on the sequences of
16S rDNA, and cultural characteristics, physiological and biochemical properties were evaluated as well. As a result, both
strains were identified as Streptomyces nashvillensis and Gordonia terrae, respectively.

Key words: Actinomycete, identification, 16S rDNA gene, phylogenetic analysis

CLC Number:

TS255.1

KONG Wangjun, JIANG Huifang, RAN Huomiao, WANG Ao, Lü Man, YANG Ke, XIN Zhihong. Isolation and Identification of Two Soil Actinomycetes ZSM-1 and ZSM-2[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201611020.

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Isolation and Identification of Two Soil Actinomycetes ZSM-1 and ZSM-2

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