Protective Effect of Zeaxanthin on SH-SY5Y Cell Injury Induced by Tunicamycin

doi:10.7506/spkx1002-6630-20171124-303

Abstract

Abstract: This study was designed to evaluate the protective effect of zeaxanthin on SH-SY5Y cell injury induced by tunicamycin (TM) in vitro. The experiment was divided into control group, model group and treatment groups with different doses of zeaxanthin. Cell viability was measured by methyl thiazolyl tetrazolium (MTT) assay, and lactate dehydrogenase (LDH) in cells and cell culture were determined by a commercial kit. The results showed that TM at concentrations over 2 μg/mL could significantly decrease cell viability (P < 0.01). In contrast to the model group, the pretreatment of zeaxanthin in the concentration range of 2–10 μmol/L could increase cell viability (P < 0.05) and suppress TM-induced LDH release in SH-SY5Y cells. The same results were obtained with the reverse sequence. TM caused cell injury by inducing endoplasmic reticulum stress. This effect was reduced or even abrogated by zeaxanthin.

Key words: zeaxanthin, tunicamycin, endoplasmic reticulum stress, SH-SY5Y cells

CLC Number:

TS201.4

WEI Jun, ZHAO Fanfan, LIU Xinjun, LAO Fengxue, HUANG Hanchang, SHANG Yinghui. Protective Effect of Zeaxanthin on SH-SY5Y Cell Injury Induced by Tunicamycin[J]. FOOD SCIENCE, 2019, 40(7): 163-168.

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Protective Effect of Zeaxanthin on SH-SY5Y Cell Injury Induced by Tunicamycin

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