Effect of Electron Beam Irradiation on Immunogenicity and Conformation of Tropomyosin from Shrimps (Solenocera melantho)

doi:10.7506/spkx1002-6630-20180125-345

Abstract

Abstract: In order to illustrate the relationship between the conformation change of shrimp tropomyosin (TM) after electron beam (EB) irradiation and its immunogenicity, TM extracted from Solenocera melantho was irradiated with different EB doses (0, 1, 3, 5, 7 and 9 kGy). The secondary structure and surface hydrophobicity of the irradiated TM were analyzed by circular dichroism and fluorescence spectroscopy, and the immunoglobulin G (IgG)-binding capacity was determined using Western blot and indirect enzyme-linked immunosorbent assay (ELISA). The results showed that EB irradiation reduced the content of TM and changed its secondary structural composition of. The α-helix content decreased, while the β-sheet and random coil contents as well as the surface hydrophobicity increased with the increase of irradiation dose; meanwhile, the IgG-binding capacity decreased. In conclusion, it is demonstrated that EB irradiation could decrease TM immunogenicity by changing its conformational structure, which provides a theoretical basis for the application of EB irradiation to decrease the potential allergenicity of Solenocera melantho meat.

Key words: Solenocera melantho, tropomyosin, electron beam irradiation, immunogenicity, protein conformation

CLC Number:

TS254.1

GUAN Aiyan, LUO Huabin, MEI Kalin, ZHANG Jinjie, XU Dalun, LOU Qiaoming, YANG Wenge, LU Tongxia. Effect of Electron Beam Irradiation on Immunogenicity and Conformation of Tropomyosin from Shrimps (Solenocera melantho)[J]. FOOD SCIENCE, 2019, 40(3): 116-121.

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Effect of Electron Beam Irradiation on Immunogenicity and Conformation of Tropomyosin from Shrimps (Solenocera melantho)

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