Determination of 5 Kinds of Illegally-added Colorants in Drink and Candy

Abstract

Abstract: A quantitative determination method for Brilliant Black PN, Fluorescein Sodium, Red 2G, Phloxine B and Rose Bengal in drink and candy was established using high performance liquid chromatography (HPLC). For sample preparation, extraction by polyamide adsorption and desorption with ethanol-ammonia solution-water (7:2:1, V/V) were carried out. An ODS C18 column (250 mm × 4.6 mm, 5μm) was used for chromatographic separation, which was eluted with methanol-0.02 mol/L ammonium acetate aqueous solution as mobile phase at 35 ℃. The linear range of the developed method was 1.0－10.0 mg/L with relative standard deviation of 1.99%－5.95% and the detection limit was 1.0 mg/kg. The average recovery rates were 82.95%－95.43%. This method is simple, accurate, and suitable for the determination of these illegally-added colorants in drink and candy.

Key words: HPLC, brilliant black PN, fluorescein sodium, red 2G, phloxine B, rose bengal

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Determination of 5 Kinds of Illegally-added Colorants in Drink and Candy

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