Abstract: Through a series of experiments, the process for producing alcohol active dry yeast (AADY) was optimized and the optimum protective agent was selected. Saccharomyces cerevisiae was inoculated at 1%－3% (V/V) into a fermentor for the optimized 40 h fermentation at 28 ℃ and 10 NL/min air flow with shaking at 200 r/min. The inlet temperature of fluidized bed was set at 90 ℃. The protective agent used for ordinary AADY was a combination of 2% Span-60 and 2% Tween-80 and the percentage of living AADY cells was up to 77.89%. The combination of 5% trehalose, 2% Span-60 and 2% Tween-80 could be used for the protection of high-quality AADY and strain conservation and keep (83.52 ± 1.87) % of AADY cells alive.

Key words: alcohol active dry yeast (AADY), drying protective agent, process