FOOD SCIENCE ›› 0, Vol. ›› Issue (): 214-217.

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Simultaneous Determination of Eight Catechins, Three Purine Alkaloids and Gallic Acid in Tea by High-performance Liquid Chromatography

  

  • Received:2010-10-29 Revised:2011-08-04 Online:2011-09-25 Published:2011-09-24

Abstract: A method was developed to determine catechins, purine alkaloids and gallic acid in tea by HPLC-PDA. The optimum condition was achieved by separation on a Welchrom C18 column kept at 30 ℃ using phosphate buffer-acetonitrile as the mobile phase at a flow rate of 1.0 mL/min through gradient elution and detection at 278 nm. This method showed a good linearity for all 12 tested compounds. The results demonstrated high accuracy and stability of this method.

Key words: high performance liquid chromatography (HPLC), catechin, methylated EGCG, purine alkaloid, gallic acid