Abstract: In this paper, immunogen OTA-BSA was prepared by EDC method and used to immunize mice for constructing a hybridoma cell line with the ability to secrete anti-OTA monoclonal antibody (anti-OTA mAb). The preparation of ascites was carried out using in vivo induction method. Immunological properties such as subclass, titer, sensitivity, affinity and specificity of the purified mAb were characterized. The mAb against OTA was type IgG1, with ELISA titer of 1: 6.4 × 105, IC50 value of 112.8 pg/mL, affinity constant (Ka) of 9.74×1011 L/mol, cross-reactivity to OTB and other fungus metabolites of 5.6% and less than 0.003%, respectively. The anti-OTA mAb was characterized with high titer, sensitivity and specificity and can thereby be used for rapid immunological detection of OTA.

Key words: ochratoxin A(OTA), monoclonal antibody, sensitivity, specificity