Isolation, Identification and Carbon Metabolic Fingerprinting Analysis of Four Pathogens Isolated from Postharvest Plum Fruit

Abstract

Abstract: Four pathogens, strain 059#, 067#, 087# and 088#, were isolated from infected plum fruit during postharvest storage. Morphological characterization and rDNA ITS analysis confirmed that the four pathogens were Gibberella moniliformis, Alternaria alternate, Penicillium oxalicum and Aspergillus tubingensis, respectively. A Biolog Microbial Identification System with FF MicroPlate was applied for carbon source utilization tests of the four pathogens based on their abilities to utilize 95 different substrates. The results of carbon metabolic fingerprinting analysis showed that all the isolated pathogens had more than 77 optimal carbon sources, 45 ones in common such as D-fructose, D-glucosamine, sucrose, sorbitol, malic acid and citric acid.

Key words: plum fruit, fungal pathogen, ITS rDNA sequence analysis, carbon metabolic fingerprinting

CLC Number:

TS201

References

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Isolation, Identification and Carbon Metabolic Fingerprinting Analysis of Four Pathogens Isolated from Postharvest Plum Fruit

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