FOOD SCIENCE ›› 2018, Vol. 39 ›› Issue (20): 112-118.doi: 10.7506/spkx1002-6630-201820017

• Bioengineering • Previous Articles     Next Articles

Morphological Characterization and rDNA ITS Sequence Analysis of Filamentous Fungi Isolated from Wallace Melon

WANG Zhiyuan1,2, YAO Ting1, FAN Yawei1, ZHANG Yan1, WANG Yousheng1,*   

  1. (1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU), Beijing 100048, China; 2. Beijing Scientific Instruments and Equipment Cooperation Service Center, Beijing 100035, China)
  • Online:2018-10-25 Published:2018-10-24

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Abstract: In this study, twelve strains of filamentous fungi were isolated from the infected Wallace melon. All the purified isolates caused the same disease symptoms when re-inoculated to healthy Wallace melon. The results of morphological characterization and rDNA internal transcribed spacer (ITS) sequence analysis showed that one strain of Epicoccum nigrum, one strain of Penicillium oxalicum, two strains of Alternaria Nees ex Wallr., three strains of Aspergillus Micheli ex Fr., and five strains of Fusarium LK. ex Fr. were isolated. E. nigrum was isolated for the first time from Wallace melon. In addition, new pathogens of Penicillium, Alternaria, Aspergillus, and Fusarium including P. oxalicum, Alternaria tenuissima, Aspergillus awamori, F. equiseti, F. verticillioides and F. avenaceum were also isolated.

Key words: Wallace melon, filamentous fungi, morphological characterization, rDNA ITS sequence analysis

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