Abstract: An ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) method was established to characterize phenolic compounds in domestic olive (Olea europaea L.) leaf. UPLC separation was performed on a Waters BEH-C18 column (10.0 mm×2.1 mm, 1.7 μm) using a mobile phase consisting of 0.1% formic acid water solution (A) and acetonitrile (B). The gradient program used was as follows: 0－10 min, 10% B－30% B; 10－12 min, 30% B－33% B; 12－17 min, 33% B－38% B. The flow rate was set at 0.25 mL/min and the detection wavelength at 280 nm. The mass spectrometer used was a Waters ACQUITY Q-TOF-MS equipped with an electrospray ionization (ESI) interface in negative mode. Fifteen phenolic compounds were identified from the extracts of domestic olive leaf by analyzing the total ion current (TIC) chromatograms and MS fragmentation patterns.

Key words: ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS), domestic olive leaf, phenolic compounds