Determination of Monosaccharide and Disaccharide in Foods by HPLC Method

Abstract

Abstract: HPLC method was established to determine the monosaccharide and disaccharide in foods. The chromatographic conditions are as belows: Waters amino chromatographic column with refractive index detector, elution influent methane:water (V/V) 85:15, flow rate 1.0ml/min and injection volume 10μl. The repeatability relative standard deviation of this method is below 5% and the recovery is over 97%. A satisfsacfory result is obtained under these optimum conditions, and the whole separation can be finished with in 20min. The results showed that this new HPLC analysis method is both correct and fast enough to be used in most kinds of foods contained monosaccharide and disaccharide.

Key words: HPLC, monosaccharide and disaccharide, foods

LIU Yu-Feng, LI Li, LI Dong, WANG Jing, FANG Xiang, WU Fang-Di. Determination of Monosaccharide and Disaccharide in Foods by HPLC Method[J]. FOOD SCIENCE, 2007, 28(3): 293-296.

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Determination of Monosaccharide and Disaccharide in Foods by HPLC Method

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