Abstract A molecularly imprinted polymer (MIP) was synthesized by bulk polymerization method using propionamide as the template, methylacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linker. Adsorption experiments were carried out on the MIP with perfect molar ratio of 1:26 between the template and the monomer. The adsorption isothermal curves of MIP and NMIP showed that the adsorption quantity of sarafloxacin in the cavity formed by MIP was higher than that of NMIP. Two kinds of binding sites were observed between the imprinted polymer and the template (sarafloxacin). Their deassiocation constants (Kd) were 0.321 μg/mL and 1.577 μg/mL, and the apparent maximum absorption capacity (Qmax) were 1.249 mg/g and 3.222 mg/g, respectively. The adsorption dynamic curve of MIP displayed that the adsorption of sarafloxacin on MIP could reach equilibrium within 7 h. Therefore, MIP has good adsorption capacity for sarafloxacin.