Abstract: A total of 123 typical strains of actinomycetes were isolated from the air in fermentation workshop and Qu workshop, pit mud and fermented grains in several distilleries of Luzhou-flavor multiple-grains using modified Gogan-I medium. Their phylogenetic analysis was carried out based on 16S rRNA gene sequence. The results showed that the 123 strains belonged to 3 genera (Streptomyces, Massilia and Nocardiopsis), with Streptomyces being the dominant one (121 strains, accounting for 98.4% of the total isolated 123 strains). The 16S rRNA sequence similarity of the isolated 121 Streptomyces strains to those of the 26 typical strains published in Genbank was more than 98%, among which 45 strains were most closely related to the typical strains S. violascens ISP 5183T and distributed in 4 sample plots. Among the 4 sample plots, the richness index and diversity index was the highest in pit mud, and the dominance index was the highest in fermented grains. The similarity index among 4 sample plots was low and special species of actinomycetes existed in every plots.

Key words: Luzhou-flavor multiple-grains liquor, actinomycetes, 16S rRNA gene sequence, phylogenetic analysis, diversity