Breeding of High-yield Surfactin-producing Strain by a Combined Mutagenesis Approach

Abstract

Abstract: In order to breed a high-yield surfactin-producing strain, Bacillus subtilis fmbJ224 was used as the start strain and mutagenized sequentially with lithium chloride (LiCl), nitrosoguanidine (NTG), ethyl methane sulphonate (EMS) and streptomycin. The preliminary and secondary screening results showed that strain LN2-3 had high ability to produce surfactin, with a yield of 239.2 mg/L, which revealed a 44.56-fold increase compared with the original strain. Meanwhile, the strain had good genetic stability. This study demonstrates that the mutation rate of the original strain could be raised greatly by the combined use of LiCl and NTG.

Key words: Bacillus subtilis, nitrosoguanidine (NTG), LiCl, combined mutagenesis, surfactin

CLC Number:

TS201.3

lu fengxia. Breeding of High-yield Surfactin-producing Strain by a Combined Mutagenesis Approach[J]. FOOD SCIENCE, 0, (): 270-276.

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