Abstract: To investigate the influence of Juglans mandshurica Oil (JMO) on antioxidant abilities in aged mice, a subacute aging mice model was built by neck back subcutaneous injection of D-galactose for 6 consecutive weeks. Using VC as positive control, JMO was given to mice by gavage at three different doses of 5, 10 mL/(kg·d) and 20 mL/(kg·d). The control and model control groups were given normal saline by gavage during that time. Total antioxidant capacity (T-AOC), catalase (CAT), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and monoamine oxidase (MAO) in the serum, liver and brain of mice were determined at the end of the last administration. The results showed that the content of T-AOC, SOD, CAT and GSH-Px in the aged model group significantly declined (P ＜0.05), but the content of MAO and MDA significantly increased as compared to the normal control group (P ＜0.05). For the aged mice treated with 10 mL(kg·d)and 20 mL/(kg·d) of JMO, the activities of SOD, CAT, GSH-Px and the content of T-AOC significantly increased (P ＜0.05) and the activity of MAO and the content of MDA significantly declined (P ＜0.05) as compared to aged control mice. These data indicated that JMO could inhibit the generation of lipid peroxides, and effectively remove free radicals, and significantly increase antioxidant ability in the body in a dose-dependent manner.

Key words: Juglans mandshurica oil, D-galactose, mice, antioxidant effect