Separation of Anthocyanins from Wild Bilberry by HPD-700 Macroporous Resin

Abstract

Abstract: In this study, the separation methods of anthocyanins from wild bilberry were investigated. The results showed that the macroporous resin HPD-7 was the optimal absorption material. The optimum conditions of adsorption and desorption were pH 2.0, anthocyanins concentration of 0.75 mg/mL, sample loading volume of 22 BV (resin bed volume) the adsorption speed of 0.5 mL/min, the 60% ethanol volume as the eluent of 5 BV, and the desorption speed of 1.5 mL/min. Under the optimal separation and purification conditions, a purplish dark powder with color value of 62.40 was achieved at the recovery rate of 86.20%.

Key words: wild bilberry, HPD-700 macroporous resin, anthocyanins, adsorption, desorption

CLC Number:

TS255.1

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