Identification of Hafnia alvei and Its Ability to Produce Biogenic Amine

Abstract

Abstract: A biogenic amine-producing strain named as C10 was isolated from chilled chicken in our laboratory. Its effect on the safety of chilled chicken was evaluated during storage. The strain was identified according to its physiological and biochemical characteristics and 16 rDNA sequence. At the same time, its ability to produce biogenic amine during culture in decarboxylase medium at 4 ℃ for 3 days was analyzed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). The strain C10 was identified as Hafnia alvei. Consistent results were obtained using TLC and HPLC, which showed that C10 could produce cadaverine, putrescine and histamine at levels of 12.28, 5.23 mg/L and 8.35 mg/L, respectively. In conclusion, Hafnia alvei is a potential hazard to the safety of chicken during refrigeration. In addition, TLC is proved to be a rapid, economical and reliable method for detecting BAs in meats.

Key words: biogenic amines, 16S rRNA, Biolog analysis, thin layer chromatography(TLC), high performance liquid chromatography (HPLC)

CLC Number:

Q939.97

References

[1] HALASZ A, BARATH A, SIMON-SARKADI L, et al. Biogenic amines and their production by microorganisms in food [J].. Trends Food Science Technology, 1994, 5: 42-48.
[2] BOVER-CID S, MIGUELEZ-ARRIZADO, M J, BECKER B, et al. Amino acid decarboxylation by Lactobacillus curvatus CTC273 affected by the pH and glucose availability. Food Microbiology, 2008, 25: 269–277.
[3] TIL H P, FALKE H E, PRINSEN M K, et al. Acute and subacute toxicity of tyramine, spermidine, spermine, putrescine and cadaverine in rats[J]. Food Chem Toxicol, 1997, 35(3–4):337–48.
[4] WARTHESEN J J, SCANLAN R A, BILLS D D, et al. Formation of heterocyclic N-nitrosamines from the reaction of nitrite and selected primary diamines and amino acids[J]. Journal of Agricultural Food Chemistry, 1975, 23:898-902.
[5] 孙长颢, 陈炳卿, 王舒然等. 胃癌高发区庄河县小咸鱼干中酪胺的致突变性[J]. 中华预防医学杂志, 1995, 29(4): 205-208.
[6] JEYA SHAKILA R, VASUNDHARA T S, KUMUDAVALLY K V. A comparison of the TLC-densitometry and HPLC method for the determination of biogenic amines in fish and fishery products[J]. Food Chemistry, 2001, 75: 255-259.
[7] DEMONCHEAUX J P, MICHEL R, MAZENOT C, et al. A large outbreak of scombroid fish poisoning associated with eating yellowfin tuna (Thunnus albacares) at a military mass catering in Dakar Senegal[J]. Epidemiology Infect, 2011, 1-5.
[8] AYESH A M, IBRAHEIM M N, EL-HAKIM A E. Exploring the contamination level by biogenic amines in fish samples collected from markets in Thuel – Saudi Arabia[J]. African Journal of Microbiology Research, 2012, 6(6): 1158-1164.
[9] PIRCHER A, BAUER F, PAULSEN P. Formation of cadaverine, histamine, putrescine and tyramine by bacteria isolated from meat, fermented sausages and cheeses[J]. European Food Research and Technology, 2007, 226: 225-231.
[10] MAH J, KIM Y, HWANG H. Inhibitory effects of garlic and other spices on biogenic amine production in Myeolchi-jeot, Korean salted and fermented anchovy product[J]. Food Control, 2009, 20(5): 449-454.
[11] BUNKOVA L, BUNKA F, KLCOVSKA P, et al. Formation of biogenic amines by Gram-negative bacteria isolated from poultry skin[J]. Food Chemistry, 2010, 121(1): 203-206.
[12] KOZACINSKI L, FLECK Z C, KOZACINSKI Z, et al. Evaluation of shelf life of pre-packed cut poultry meat[J]. VETERINARSKI ARHIV, 2012, 82(1): 47-58
[13] 赵剑峰张哲等. 冷链物流管理分析及促进中国食品冷链物流发展的政策建议[J]. 世界农业, 2008, 9: 40-43.
[14] CARELLI D, CENTONZE D, PALERMO C, et al. An interference free amperometric biosensor for the detection of biogenic amines in food products[J]. Biosensors and Bioelectronics, 2007, 23(5): 640-647.
[15] VECIANA-NOGUES M T, BOVER-CID S, MARINE-FONT A, et al. Biogenic amine production by Morganella morganii and Klebsiella oxytoca in tuna[J]. European Food Research and Technology, 2004, 218(3): 284-288.
[16] BOVER-CID S, HOLZAPFEL W H. Improved screening procedure for biogenic amine production by lactic acid bacteria[J]. International Journal of Food Microbiology, 1999, 53: 33–41.
[17] 李苗云. 冷却猪肉中微生物生态分析及货架期预测模型的研究[D]. 南京: 南京农业大学, 2006.
[18] 房亮, 李田. 1株蜂房哈夫尼亚菌的分离与鉴定[J]. 中国卫生检验杂志, 2005, 15(2): 235.