FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (7): 270-274.doi: 10.7506/spkx1002-6630-201307057

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in vitro Immunoregulatory and Antitumor Activity of Sulfated Polysaccharides from Sargassum kjellmanianum

MA Wei-wei,LI Li,ZHOU Ge-fei*   

  1. College of Life Science, Yantai University, Yantai 264005, China
  • Received:2012-05-27 Revised:2013-02-28 Online:2013-04-15 Published:2013-03-20
  • Contact: ZHOU Ge-fei E-mail:gefeizhou@hotmail.com
  • Supported by:
    Natural Science Foundation of Shandong Province: separation and activity study of the lead compounds in Sargassum Kjellmanianum

Abstract: The in vitro immune-regulatory and anti-tumor activities of sulfated polysaccharides from Sargassum kjellmanianum (SKP) were investigated in this study. Polysaccharides were first extracted from Sargassum kjellmanianum by hot water. Crude extract was then treated with TCA to remove proteins and dialyzed to remove small molecule impurities. Three different types of polysaccharides, SKP1, SKP2 and SKP3, were collected through DEAE-52 ion exchange column. Chemical analysis showed that the polysaccharides were composed of fucose, galactose, xylose and mannose. All three types of polysaccharides were sulfated. Sulfate group accounted for 3.4%, 25.6% and 29.9% in SKP1, SKP2 and SKP3, respectively. SKP2 and SKP3 significantly enhanced immune function of immunocytes, and SKP2 increased the proliferation rate of spleen lymphocytes and peritoneal macrophages up to 53.56% and 51.41%. Cell culture showed that all SKP1, SKP2 and SKP3 inhibited the proliferation of HT-29 and HeLa cells, showing their anti-tumor activity.

Key words: Sargassum kjellmanianum, sulfated polysaccharides, in vitro immune, anti-tumorigenesis

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