Abstract:

One-factor-at-a-time and orthogonal array methods were used to investigate the optimal ultrasonic-assisted extraction process of sulfated polysaccharides from Eucheuma striatum (ESSP) in this work. Results indicated that the optimal values of technological parameters, including ultrasonic treatment time and power and material/liquid ratio for achieving a maximum sulfated polysaccharide yield of 48.14% with an increased of 12.08% compared with that given by hot water extraction were 20 min, 150 W and 1:90 (g/mL), respectively. The dried crude product obtained using the optimized ultrasonic-assisted extraction process exhibited polysaccharide and sulfate group contents of 96.94% and 19.66%, respectively. Neither protein nor nucleic acid was found in ESSP obtained using hot water extraction or this method, which contained characteristic peaks of polysaccharides and sulfate groups shown in IR spectrum.

Key words: Eucheuma striatum, sulfated polysaccharides, ultrasonic, orthogonal array design