Vitamin, amino acid, mineral substance, crude protein, crude fat and crude fiber contents in fruit body, mycelia and fermentation broth of Lyophyllum decastes were determined using relevant Chinese national standard methods, and the method stipulated in Technical Standards Testing & Assessment of Health Food (2003 Version) of the Chinese ministry of health was used for determining crude polysaccharides in these materials. Results showed that fruit body, mycelia and fermentation broth of Lyophyllum decastes revealed crude protein contents of 21.4%, 28.3% and 0.084%, crude fat contents of 1.44%, 2.78% and 0.11%, carbohydrate contents of 53.04%, 54.7% and 2.82%, crude polysaccharide contents of 3.55%, 1.77% and 0.12% , total amino acid contents of 16.82, 19.8 and 0.088 g/100 g, amino acid kinds of 17, 18 and 6, lysine contents of 1.13, 1.18 and 0 g/100 g, vitamin kinds of 5, 5 and 4, niacin contents of 21.2, 25 and 0.084 mg/100 g, mineral substance kinds of 7,5 and 1, and selenium contents of 0.013, 0.080 and 0 mg/kg, respectively. The contents of crude fiber in fruit body and mycelia of Lyophyllum decastes were 9.52% and 5.32%, whereas no crude fiber was detected in fermentation broth of Lyophyllum decastes. These results indicate that fruit body, mycelia and fermentation broth all have a high utilization value due to their abundant nutrition.