HRCA-based Strip Test for Detection of Transgenic Components in Food

doi:10.7506/spkx1002-6630-201006063

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (6 ): 263-266.doi: 10.7506/spkx1002-6630-201006063

• Analysis & Detection • Previous Articles Next Articles

HRCA-based Strip Test for Detection of Transgenic Components in Food

HAO Zhen-ming1，ZHAO Xin1，WU Xiao-huai2

1. Beijing Food Safety Administration, Beijing 100080, China ；
2. Beijing Municipal Center for Food Safety Monitoring, Beijing 100041, China)

Received:2010-01-25 Online:2010-03-15 Published:2010-12-29
Contact: HAO Zhen-ming1 E-mail:haovista@126.com

Abstract

Abstract:

This study aimed to develop a strip test method based on HRCA technique for simultaneous qualitative detection of various transgenic components in food. A mixture of transgenic and nontransgenic soy bean and transgenic maize and potato in some proportion was prepared to evaluate the effectiveness of nitrocellulose membrane strips developed to immobilize specific probes in detecting transgenic components in reaction products obtained through linkage and rolling-cycle amplification. Results showed that nitrocellulose membrane strips carrying specific probes achieved 100% accuracy in detection of pairwise mixtures or full mixture of the above transgenic components or each of them and gave a detection level of 100 pg/μL, which meets the requirements of routine detection. The developed nitrocellulose membrane strips carrying specific probes may provide an effective tool for detection of transgenic components in food.

Key words: transgenic food, rolling circle amplification, test strip

CLC Number:

Q503

HAO Zhen-ming1，ZHAO Xin1，WU Xiao-huai2. HRCA-based Strip Test for Detection of Transgenic Components in Food[J]. FOOD SCIENCE, 2010, 31(6 ): 263-266 https://doi.org/10.7506/spkx1002-6630-201006063

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HRCA-based Strip Test for Detection of Transgenic Components in Food

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