Abstract:

This study aimed to develop a strip test method based on HRCA technique for simultaneous qualitative detection of various transgenic components in food. A mixture of transgenic and nontransgenic soy bean and transgenic maize and potato in some proportion was prepared to evaluate the effectiveness of nitrocellulose membrane strips developed to immobilize specific probes in detecting transgenic components in reaction products obtained through linkage and rolling-cycle amplification. Results showed that nitrocellulose membrane strips carrying specific probes achieved 100% accuracy in detection of pairwise mixtures or full mixture of the above transgenic components or each of them and gave a detection level of 100 pg/μL, which meets the requirements of routine detection. The developed nitrocellulose membrane strips carrying specific probes may provide an effective tool for detection of transgenic components in food.

Key words: transgenic food, rolling circle amplification, test strip