FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (6): 145-147.doi: 10.7506/spkx1002-6630-201006032

• Analysis & Detection • Previous Articles     Next Articles

Application of Luminescent Vibrio fischeri to Rapid Determination of Melamine in Liquid Milk

ZHANG Guo-hui1,2,ZHAO Ji1,*,SHAO Yu-qin1,YU Jing-li1,LIU Fang1   

  1. 1. College of Environment and Resources, Inner Mongolia University, Hohhot 010021, China;
    2. Hohhot Environmental Science Research Institute, Hohhot 010030, China
  • Received:2009-04-27 Online:2010-03-15 Published:2010-12-29
  • Contact: ZHAO Ji1 E-mail:nmgzhaoji@yahoo.com

Abstract:

A method based on the toxicity effect of melamine on luminescent bacterium Vibrio fisheri measured using Skalar ToxTracer system was developed for determining melamine in liquid milk. Results showed that there was a positive correlation between inhibitory rate of melamine against luminescent intensity of Vibrio fisheri and melamine concentration in aqueous solution with a correlation coefficient ranging within 0.83-0.99, whereas inhibitory rate of melamine against luminescent intensity of Vibrio fisheri was negatively correlated with melamine concentration in liquid milk, and the correlation coefficient was between 0.79 and 0.94. This method, thanks to the characteristics of rapidity, simplicity, sensitivity and low cost, is expected to gain an extensive application to food safety detection.

Key words: Vibrio fischeri, melamine, milk

CLC Number: