Abstract:

Cellulase hydrolysis followed by ultrasonic-assisted extraction was adopted to extract crude total flavonoids from Flos Chrysanthemi Indici. The optimal cellulase hydrolysis and ultrasonic-assisted extraction processes were both investigated by orthogonal array design. Besides, the purification of the crude total flavonoids with D-101 macroporous resin column was explored. Results showed that the optimal cellulase hydrolysis could be achieved via hydrolysis for 6 h at 40 ℃, pH 6.0, 1:10 material/liquid ratio (g/mL) and 1.5% enzyme dose, and the use of 70% ethanol solution to extract the cellulase hydrolysate of Flos Chrysanthemi Indici for 0.5 h resulted in an optimal extraction of total flavonoids. The purification process consisting of adsorption of total flavonoids onto D-101 macroporous resin column at natural flow rate, washing of the column with water for the removal of impurities and desorption with 60% ethanol gave a recovery and purity of total flavonoids of 7% and more than 50%, respectively.

Key words: flavonoid, ultrasonic, cellulase, macroporous resin, Flos Chrysanthemi Indici