FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (6): 48-51.doi: 10.7506/spkx1002-6630-201006011

• Processing Technology • Previous Articles     Next Articles

Enzymatic Hydrolysis Followed by Ultrasonic-assisted Extraction and Macroporous Resin Adsorption for Purification of Total Flavonoids from Flos Chrysanthemi Indici

YAO Chao1，YU Hai-ning2，RUAN Hui1，HE Guo-qing1，SHEN Sheng-rong1,*   

  1. 1. School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, China；
    2. College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China
  • Received:2009-05-04 Revised:2009-10-23 Online:2010-03-15 Published:2010-12-29
  • Contact: SHEN Sheng-rong1 E-mail:shrshen@zju.edu.cn

RichHTML

0

PDF (PC)

380

Abstract:

Cellulase hydrolysis followed by ultrasonic-assisted extraction was adopted to extract crude total flavonoids from Flos Chrysanthemi Indici. The optimal cellulase hydrolysis and ultrasonic-assisted extraction processes were both investigated by orthogonal array design. Besides, the purification of the crude total flavonoids with D-101 macroporous resin column was explored. Results showed that the optimal cellulase hydrolysis could be achieved via hydrolysis for 6 h at 40 ℃, pH 6.0, 1:10 material/liquid ratio (g/mL) and 1.5% enzyme dose, and the use of 70% ethanol solution to extract the cellulase hydrolysate of Flos Chrysanthemi Indici for 0.5 h resulted in an optimal extraction of total flavonoids. The purification process consisting of adsorption of total flavonoids onto D-101 macroporous resin column at natural flow rate, washing of the column with water for the removal of impurities and desorption with 60% ethanol gave a recovery and purity of total flavonoids of 7% and more than 50%, respectively.

Key words: flavonoid, ultrasonic, cellulase, macroporous resin, Flos Chrysanthemi Indici

CLC Number: