FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (6): 52-54.doi: 10.7506/spkx1002-6630-201006012

• Processing Technology • Previous Articles     Next Articles

Extraction and Determination of Arachidonic Acid in Mnium spinosum

WANG Gui-hua,XIE Shu-lian*   

  1. School of Life Science and Technology, Shanxi University, Taiyuan 030006, China
  • Received:2009-05-25 Revised:2009-10-23 Online:2010-03-15 Published:2010-12-29
  • Contact: XIE Shu-lian* E-mail:xiesl@sxu.edu.cn

Abstract:

Mnium spinosum (Voit) Schwagr was extracted with different organic solvents in Soxhlet extractor. The resulting extracts were then purified by silica column chromatography for analyzing arachidonic acid (AA) content by HPLC. Results showed that ethanol extract of Mnium spinosum (Voit) Schwagr exhibited an AA content of 6.5%, while no AA wad detected in petroleum ether extract and n-hexane extract of Mnium spinosum (Voit) Schwagr. Thus, ethanol is superior to other two solvents for extracting considerable AA from Mnium spinosum (Voit) Schwagr.

Key words: arachidonic acid, Mnium spinosum (Voit) Schwagr, high performance liquid chromatography (HPLC), column chromatography

CLC Number: