Isolation and Purification of Anthocyanin from Blueberry by Sequential Medium Pressure Column Chromatography on Macroporous Resin and Sephadex LH-20

doi:10.7506/spkx1002-6630-201801018

Abstract

Abstract: In this study, the anthocyanins of blueberry were isolated and purified by sequential medium pressure column chromatography using macroporous resin and Sephadex LH-20. The static adsorption and desorption of blueberry anthocyanins on six different types of resins was compared, and the separation process of anthocyanins by macroporous resin adsorption chromatography was optimized. The results showed that D101 resin gave the best separation efficiency and its adsorption behavior obeyed a multi-molecular layer adsorption. Under the following conditions: column pressure 1 MPa, temperature 25 ℃, anthocyanins concentration 0.073 mg/mL, and elution with 80% ethanol at a flow rate of 5 mL/min, the purity of anthocyanins increased by 13.67 folds from 5.53% to 75.58%. A purified anthocyanin fraction was obtained by subsequent Sephadex LH-20 column chromatography, which was identified as cyanidin-3-O-glucoside using highperformance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESI-MS) with a purity of 90.88%.

Key words: blueberry, medium pressure column chromatography, anthocyanins, macroporous resin

CLC Number:

TS201.1

YU Zeyuan, ZHAO Jianhui, LI Xingguo, XU Yaqin, TANG Xue, YANG Yu. Isolation and Purification of Anthocyanin from Blueberry by Sequential Medium Pressure Column Chromatography on Macroporous Resin and Sephadex LH-20[J]. FOOD SCIENCE, 2018, 39(1): 118-123.

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Isolation and Purification of Anthocyanin from Blueberry by Sequential Medium Pressure Column Chromatography on Macroporous Resin and Sephadex LH-20

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