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Optimization of Microwave-Assisted Extraction of Pectic Polysaccharides from Agave sisalana Perrine by Response Surface Methodology

YAO Xian-chao,ZHANG Xue-hong,LIANG Yu-shi,ZHOU Hong,LIN Cui-wu   

  1. 1. School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China;
    2. School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China;
    3. Department of Facility and Laboratory Management, Guangxi University for Nationalities, Nanning 530006, China
  • Online:2013-09-25 Published:2013-09-27

Abstract:

The microwave-assisted extraction of pectic polysaccharides from sisal wastes left over after fiber production
was investigated. Response surface methodology (RSM) based on a three-level, three-variable Box-Behnken design was
applied to optimize three extraction conditions including microwave output power, extraction time and solvent pH. The
optimum extraction conditions were found to be 534 W, 9.8 min and pH 1.3. The yield of ASP (pectic from Agave sisalana
Perrine) under the optimized conditions was 6.10% and galacturonic acid content and esterification degree of the extract
were 75.3% and 72.09%, respectively.

Key words: response surface methodology, Agave sisalana, pectic, microwave, extraction