Abstract:

The purification of total flavonoids from cinnamon was studied using macroreticular resin. Critical process
variables that influence the static and dynamic adsorption and desorption of total flavonoids were selected and optimized.
HPD-500 type macroreticular resin was found to be optimal for purifying total flavonoids from cinnamon. The optimal
experimental conditions for static adsorption and desorption were determined as 6 h, 2 h, 0.8 mg/mL, 6.0 and 80% ethanol
for adsorption time, desorption time, sample concentration, sample pH and desorption solvent, respectively. The optimal
experimental conditions for dynamic adsorption and desorption were loading of 1.2 mg/mL sample solution at pH 6.0 with
a flow rate of 1.5 mL/min and complete desorption with 45 mL of 80% ethanol at a flow rate of 2.0 mL/min. The content of
total flavonoids in purified products under the optimized conditions was 91.81%.

Key words: cinnamon, total flavonoids, separation and purification, macroreticular resin