Separation, Purification and Biochemical Properties of Exopolysaccharides from Weissella confusa

doi:10.7506/spkx1002-6630-20190623-270

Abstract

Abstract: A strain of lactic acid bacteria (LAB) with high exopolysaccharides (EPS)-producing ability was isolated from naturally fermented melon. The strain was identified as Weissella according to its physiological and biochemical properties. In addition, the chemical composition, solubility, water-holding capacity, emulsifying property, antibacterial property and antioxidant activity of the purified EPS from this strain were determined. The results showed that the total sugar, protein and uronic acid contents were (93.67 ± 2.08)%, (0.32 ± 0.01)% and (6.01 ± 0.34)%, respectively. The dissolution percentage and water holding capacity of the EPS were (98.78 ± 1.37)% and (426.03 ± 7.26)%, respectively. It exhibited good emulsifying, bacteriostatic and in vitro antioxidant properties in a concentration-dependent manner.

Key words: Weissella confusa, exopolysaccharide, separation and purification, biochemical properties

CLC Number:

Q939.97

JIANG Jing, DU Renpeng, GUO Shangxu, ZHAO Dan. Separation, Purification and Biochemical Properties of Exopolysaccharides from Weissella confusa[J]. FOOD SCIENCE, 2020, 41(1): 9-15 https://doi.org/10.7506/spkx1002-6630-20190623-270

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Separation, Purification and Biochemical Properties of Exopolysaccharides from Weissella confusa

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