FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (6): 163-169.doi: 10.7506/spkx1002-6630-20190811-124

• Bioengineering • Previous Articles     Next Articles

Effect of Exopolysaccharides of Lactobacillus helveticus MB2-1 on Growth Characteristics of Ten Probiotics

HUANG Rong, ZHANG Xueliang, HAN Shuo, ZHOU Ziwen, MO Qiaoya, DONG Mingsheng, RUI Xin, ZHANG Qiuqin, CHEN Xiaohong, LI Wei   

  1. (College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China)
  • Online:2020-03-25 Published:2020-03-23

Abstract: This study evaluated the effects of the exopolysaccharides (EPS) produced by Lactobacillus helveticus MB2-1 and its purified components on the growth characteristics of probiotics in order to gain further insights into the probiotic function of the EPS of L. helveticus MB2-1. The optical density (OD600 nm) values of ten probiotics and two pernicious strains continuously cultured in a medium with EPS or EPS-2, the most abundant component separated and purified from EPS, as the sole carbon source were determined. The residual amounts of total EPS and the amounts of short-chain fatty acids (SCFA) produced in 10 probiotics during 48 h of culture were measured by high performance liquid chromatography (HPLC). The results showed that the 10 probiotics could grow and reproduce at different rates in the medium with EPS or EPS-2 as the sole carbon source. Among them, Enterococcus faecium MN-1grown in EPS showed a maximum growth rate of 0.225 h-1 at 11.56 h. The growth of the two pernicious strains was inhibited to a certain extent compared with normal glucose medium. Lactic acid was produced in larger amounts than other SCFA. L. paracasei was the most efficient producer of lactic acid, and at 48 h of fermentation, the lactic acid concentration reached 57.6 mmol/L, which increased by 121.7% as compared with 26.1 mmol/L at the beginning of fermentation. This study demonstrated that the EPS derived from L. helveticus MB2-1 is a good carbon source for the ten probiotics and can be used as potential prebiotics to regulate intestinal health in humans and animals.

Key words: Lactobacillus helveticus MB2-1, exopolysaccharides, growth curve, short-chain fatty acids, probiotic function

CLC Number: