Comparison of Resistance of Bifidobacterium animalis Strains Isolated from Infants to Simulated Gastrointestinal Stress and Effect of Selected Strain on Activities of Immune Cells in Vitro

doi:10.7506/spkx1002-6630-20181017-178

Abstract

Abstract: Four strains of Bifidobacterium animalis subsp. lactis isolated from infants were evaluated for tolerance to acids, bile salts and simulated digestive tract environment, adhesiveness to Caco-2 cells and inhibitory effects on pathogen adhesion. In addition, the ability to synthesize exopolysaccharides was determined and the relationship between tolerance and adhesion was discussed. Strain H15-2, with good environmental tolerance and adhesiveness, was further evaluated for its immunomodulatory activity by considering splenocyte proliferation, energy metabolism level in macrophages and macrophage phagocytosis of neutral red. The results showed that B. animalis H15-2 had strong tolerance to simulated gastrointestinal environment, high adhesion ability to Caco-2 cells, and good regulatory effects on the activities of immune cells. This study indicates that B. animalis H15-2 has great potential for application development.

Key words: Bifidobacterium animalis, tolerance, adhesion, exopolysaccharides, immunoregulation

CLC Number:

TS201.3

WAN Feng, SUN Sirui, HOU Yujia, ZHAO An, ZHANG Sheng, MENG Xiangchen. Comparison of Resistance of Bifidobacterium animalis Strains Isolated from Infants to Simulated Gastrointestinal Stress and Effect of Selected Strain on Activities of Immune Cells in Vitro[J]. FOOD SCIENCE, 2019, 40(22): 118-125.

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Comparison of Resistance of Bifidobacterium animalis Strains Isolated from Infants to Simulated Gastrointestinal Stress and Effect of Selected Strain on Activities of Immune Cells in Vitro

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