FOOD SCIENCE

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Development and Application of a Method for Rapid Detection of Beef Content in Commercial Beef Products

LIU Bin,YANG Jin-yi,XU Wan,SUN Yuan-ming   

  1. 1. Key Laboratory of Risk Assessment to Post-harvested Product Storage, Ministry of Agriculture, Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China;
    2. College of Animal Science, South China Agricultural University, Guangzhou 510642, China
  • Online:2013-12-25 Published:2014-01-03

Abstract:

In this study, a SYBR Green Ⅰ-based real-time quantitative RT-PCR (qRT-PCR) was established to rapidly determine
bovine DNA in commercial beef meatballs. Two pairs of primers for bovine DNA (SB and TB) and the primer pair TM for
mammalian DNA were designed. A standard calibration curve of the logarithm of beef content against cycle threshold (Ct) was
plotted. The percentage of beef meat in meatball samples was calculated based on Ct(SB)–Ct(TM) or Ct(TB)–Ct(TM), minusing
Ct (TM) with Ct (SB) and Ct (TB), respectively. The limit of detection (LOD) was 0.1% for beef content, and the linear range for
quantitative determination was in the range of 0.1% to 90%.

Key words: beef, identification, fluorescent quantitative PCR, SYBR-GreenⅠ