Abstract: In this work, molecularly imprinted polymers (MIPs) for the numb-taste compounds were prepared by using the molecular structure analogs of acid amides (LM) as dummy template molecule. In order to optimize the preparation conditions of the dummy molecularly imprinted polymer for the numb-taste components of Zanthoxylum, UV spectra was applied to study the effect intensity and the optimal concentration ratio between the dummy template and the functional monomer. With ethylene glycol dimethacrylate (EDMA) as the cross-linker, acetonitrile as the pore-foaming agent, 2,2-azobisisobutyronitrile(AIBN) as the initiator, 16 kinds of MIPs were prepared by method of bulk polymerization. Then a best kind of MIPs was chosen out from the several kinds of MIPs to adsorb the numb-taste components from the extraction liquor of Zanthoxylum bungeanum, the result showed that the selected MIPs had the specific recognition and selectivity for the numb-taste components of Zanthoxylum, the relatively pure numb-taste components of Zanthoxylum can be obtained by the MIPs. The results of the research showed that with the dummy template (LM), the MIPs could achieve a good imprinting effect for the numb-taste components. The research also provides an available method for the molecular imprinting technique of substances with similar structural characteristics.

Key words: numb-taste components of Zanthoxylum, molecular imprinting polymers, preparation, dummy template