Abstract: Molecularly imprinted polymers (MIPs) were prepared by bulk polymerization with cyromazine as the virtual template molecule, methacrylic acid (MAA) as the functional monomer, trimethylolpropane trimethacrylate (TRIM) as the ternary crosslinking agent, and acetonitrile as the porogen. The optimum molar ratio of cyromazine to MAA to TRIM was determined to be 1:4:4. The as-prepared polymer was evaluated and characterized by equilibrium adsorption test, infrared spectroscopy, and scanning electron microscopy (SEM). The adsorption performance test and the Scatchard equation showed that the MIP had the best adsorption capacity toward prometryn. Two types of adsorption sites existed, and the maximum apparent binding capacity was 3.13 mg/g. A molecularly imprinted solid phase extraction (MISPE) column was prepared with the MIP, and the elution conditions were optimized. On this basis, a method for the determination of four triazine pesticides in cucumber, apple and corn was established by combining high performance liquid chromatography (HPLC). The results showed that 10% acetic acid-methanol was the best eluent for the target pesticides. The detection limits of this method were 0.01–0.03 ng/mL, and the average recoveries were 81.5%–104.3%, with relative standard deviations (RSDs) of 1.3%–5.8% (n = 3). The method could meets the requirements for the detection of pesticides with greatly improved efficiency and accuracy, which will provide theoretical support for the detection of triazine pesticide residues.

Key words: triazine pesticides; molecularly imprinted polymer; dummy template; solid phase extraction