Effect on blood lipid and hepatoprotective efficacy of flavonoids from Sonchus oleraceus L in hyperlipidemic mice

Abstract

Abstract: To observe the effect of flavonoids from Sonchus oleraceus L on the blood lipid and its hepatoprotective efficacy in hyperlipidemic mice. We selected sixty male ICR mice, which were randomly divided into six groups: normal control group, high fat model group, positive control group (Simvastatin,10 mg·kg-1), low dose group of FSOL (50mg·kg-1), middle does group of FSOL (100 mg·kg-1), high does group of FSOL (200 mg·kg-1). The normal control group was fed with basic diet, and the other groups were fed with high fat diet. After 5 weeks, serum TC, TG, LDL-C, HDL-C, ALT, AST, MDA, SOD and liver TC, TG, MDA, SOD were detected, and morphological changes in liver of mice were observed. The results show that FSOL could prevent the obesity induced by high fat diet, the serum TC, TG, LDL-C, ALT, AST, MDA and Atherosclerosis index(AI) levels were also reduced. In addition, the levels of HDL-C and SOD have increased to varying degrees. It shows that FSOL has better hypolipidemic and hepatoprotective effects on hyperlipidemia mice, and the reason may be related with liver lipid metabolism improvement and antioxidant function enhancement. This provides a theoretical basis for the development and utilization of FSOL.

Key words: Flavonoids from Sonchus oleraceus L, Hyperlipoidemia, Lipid metabolism, Hepatoprotective efficacy

CLC Number:

R285.5

References

[1] 曹宁, 郭文洁, 唐佳喻, 等. 乌药叶总黄酮对高脂血症脂肪肝小鼠模型的降脂作用[J]. 中药新药与临床药理, 2011, 22(2):149-153. DOI: 10.19378/j.issn.1003-9783.2011.02.006.
[2] 卢秋玉, 陈晓宇, 申庆荣, 等. 木棉花总黄酮降血脂作用及其机制研究[J]. 中药药理与临床, 2016(1):88-90. DOI: 10.13412/j.cnki.zyyl.2016.01.025.
[3] 孙天宇. 沙棘叶黄酮的提取分离及降血脂作用小鼠试验的研究[D]. 黑龙江八一农垦大学, 2012:36-40.
[4] 卫娜, 徐勇, 袁娟, 等. 柑橘中提取的柑橘黄酮调节高脂血症的研究[J]. 食品与发酵科技, 2014, 50(5):5-8. DOI: 10.3969/j.issn.1674-506X.2014.05-002.
[5] Chang J J, Chung D J, Li Y J, et al. Solanum nigrum polyphenol extracts inhibit hepatic inflammation, oxidative stress, and lipogenesis in high-fat-diet-treated mice[J]. J Agric Food Chem, 2017, 65(42):9255-9265. DOI: 10.1021/acs.jafc.7b03578.
[6] 李倩倩, 王艳, 罗旭, 等. 大豆低聚糖及其降血脂作用研究进展[J]. 核农学报, 2016, 31(9):1788-1793. DOI: 10.11869/j.issn.100-8551.2017.09.1788.
[7] 邓丽红, 谢臻, 陈勇, 等. 苦菜药理作用及工艺优化研究进展[J]. 大众科技, 2017, 19(10):24-28. DOI: 10.3969/j.issn.1008-1151.2017.10.009.
[8] 权美平. 微波辅助提取苦菜总黄酮的工艺优化[J]. 现代食品科技, 2013, 29(5):1065-1067. DOI: 10.13982/j.mfst.1673-9078.2013.05.002.
[9] 翟硕莉, 张秀丰. 超声波辅助水提苦菜中黄酮类物质的工艺[J]. 食品研究与开发, 2014, 35(24):63-65. DOI: 10.3969/j.issn.1005-6521.2014.24.017.
[10] 安卓, 贾昌喜. 大孔树脂分离纯化苦苣菜黄酮的工艺研究[J]. 食品与机械, 2012, 28(3):122-125.
[11] 卢赛赛, 许凤, 王鸿飞, 等. 杨梅叶中总黄酮提取及其抗氧化能力研究[J]. 果树学报, 2015, 32(3):460-468.
[12] 杨佩磊, 蒋剑平, 李全清,等. 胡柚皮黄酮对高脂血症大鼠的降血脂作用研究[J]. 中国中药杂志, 2017, 42(5):936-943. DOI: 10.19540/j.cnki.cjcmm.20170121.008.
[13] 郭苗, 杨小兰. 酒花多酚对高脂血症小鼠的降脂与抗氧化作用[J]. 食品科学, 2015, 36(3):183-187. DOI: 10.7506/spkx1002-6630-201503035.
[14] 朱振元, 李楠, 张静怡,等. 雪莲果多糖降血脂及降血糖活性的研究[J]. 现代食品科技, 2017, 33(5):39-46. DOI: 10.13982/j.mfst.1673-9078.2017.5.007.
[15] 张金堂, 沙迪, 夏广军,等. 东北山核桃壳黄酮对小鼠氧化损伤的防御作用[J]. 食品科学, 2018, 39(5):252-257. DOI: 10.7506/spkx1002-6630-201805038.
[16] 蔡天娇, 王瑞珍, 魏君慧, 等. 白桦脂酸与红枣总三萜酸对小鼠酒精肝损伤的保护作用[J]. 食品科学, 2018, 39(11):191-195. DOI: 10.7506/spkx1002-6630-201811030.
[17] Reza M A, Hossain M A, Damte D, et al. Hypolipidemic and Hepatic Steatosis Preventing Activities of the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Higher Basidiomycetes) Ethanol Extract In Vivo and In Vitro[J]. International Journal of Medicinal Mushrooms, 2015, 17(8):723-734. DOI: 10.1615/IntJMedMushrooms.v17.i8.30.
[18] Chernukha I M, Fedulova L V, Kotenkova E A, et al. Hypolipidemic and anti-inflammatory effects of aorta and heart tissues of cattle and pigs in the atherosclerosis rat model[J]. Animal Science Journal, 2018, 89(5):784-793. DOI: 10.1111/asj.12986.
[19] Liu M, Tan J J, He Z Y, et al. Inhibitory effect of blue honeysuckle extract on high-fat-diet-induced fatty liver in mice[J]. Animal Nutrition, 2018, 4(3):288-293. DOI: 10.1016/j.aninu.2018.06.001.
[20] He D Y, Zhang P, Sai X, et al. Hypolipidemic Activity of Camellia euphlebia, Flower Extract in High–fat-fed Mice[J]. Plant Foods for Human Nutrition, 2017, 72(4):372-379. DOI: 10.1007/s11130-017-0632-3.
[21] 张利, 李典鹏, 杨子明, 等. 番茄皂苷A对小鼠血脂及肝脏脂肪的作用[J]. 广西植物, 2018, 38(5):583-589. DOI: 10.11931/guihaia.gxzw201711014.
[22] Chung S I, Kim T H, Rico C W, et al. Effect of instant cooked giant embryonic rice on body fat weight and plasma lipid profile in high fat-fed mice[J]. Nutrients, 2014, 6(6):2266-2278. DOI: 10.3390/nu6062266.
[23] Fang Y Q, Sang H, Yuan N, et al. Ethanolic extract of propolis inhibits atherosclerosis in ApoE-knockout mice[J]. Lipids in Health & Disease, 2013, 12(1):1-6. DOI: 10.1186/1476-511X-12-123.
[24] 张丽静. 芍药苷对小鼠高脂饮食诱导的非酒精性脂肪肝的保护作用及机制研究[D]. 武汉大学, 2016:9-14.
[25] He Q F, Li Y J, Li H, et al. Hypolipidemic and antioxidant potential of bitter gourd (Momordica charantia L.) leaf in mice fed on a high-fat diet[J]. Pakistan Journal of Pharmaceutical Sciences, 2018, 31(5):1837-1843.
[26] 杨艳杰, 何弘水. 猕猴桃根提取物抗氧化及保肝作用的研究[J]. 安徽农业科学, 2008, 36(19):8059-8061. DOI: 10.3969/j.issn.0517-6611.2008.19.077.
[27] 孙朦, 王鸿飞, 王凯凯,等. 辣木叶黄酮对小鼠血脂调节作用研究[J]. 核农学报, 2018, 32(08):1597-1602. DOI: 10.11869/j.issn.100-8551.2018.08.1597.
[28] 郭晓娜, 刘现辉, 闫春生, 等. 桑椹多糖对高脂血症大鼠血脂代谢、血液流变学及氧自由基的影响[J]. 中国老年学, 2016, 36(18):4421-4423. DOI: 10.3969/j.issn.1005-9202.2016.18.008.
[29] 王志华, 郝峻巍, 郭君伟, 等. 大豆异黄酮对高脂去卵巢兔血脂及血清SOD、MDA的影响[J]. 山西医科大学学报, 2007, 38(4):292-294. DOI: 10.3969/j.issn.1007-6611.2007.04.002.
[30] Yang H J, Zhang X H. Polysaccharides from Polygonatum odoratum strengthen antioxidant defense system and attenuate lipid peroxidation against exhaustive exercise-induced oxidative stress in mice[J]. Tropical Journal of Pharmaceutical Research, 2017, 16(4):795-801. DOI: 10.4314/tjpr.v16i4.8.
[31] 李林子, 吕圭源, 陈素红, 等. 三七叶总皂苷对高脂血症大鼠血脂、肝功能及脂质过氧化的影响[J]. 中国现代应用药学, 2014, 31(6):662-666. DOI: 10.13748/j.cnki.issn1007-7693.2014.06.005.