Abstract: Abstract: In order to establish a simple, rapid and accurate analytical method for the determination of oxidized triacylglycerol polymers (TGP) in oils and fats, non-polar components (Non-PC) and polar components (PC) such as oxidized triacylglycerol oligomers (TGO), oxidized triacylglycerol dimers (TGD) , oxidized triacylglycerol monomers (ox-TGM), diacylglycerls (DG) and free fatty acids (FFA) were separated by high performance liquid chromatography and detected by evaporative light scattering detector with two sequential columns (forward silica column connected with gel column). DG was used as external standard to quantitatively determine TGP in oils combined with area normalization method.The eluent used was a gradient of petroleum ether (A) and tetrahydrofuran (B). Elution was performed at a solvent flow rate of 0.7 ml/min with a linear gradient as follows: 0 min 5% B, 20 min 15% B, 30 min 30% B, 35 min 5% B, keeping these conditions during 50 min. The results showed that the TGP provides nonlinear response curves, with correlation coefficients (R2) was 0.9995, the limit of detection (LOD) of TGD was 156 mg/L, and the limit of quantitation (LOQ) was 267 mg/L. The recoveries of the spiked DG of the method were between 95% and 99% with the relative standard deviation (RSD) less than 2%. Moreover, the method provided good repeatability for TGP in the detection of oil and fat with RSD less than 3%. This method can quantify the content of TGP in oils and fats within 1 h, which is easy to operate and accuracy quantitative, and saves experimental materials.

Key words: Key words: oxidized triacylglycerol polymer, polar component, two sequential columns, high performance liquid chromatography