Preparation of antibacterial membrane of zinc containing and ε-polylysine and study on its antibacterial and physical properties

Abstract

Abstract: In this paper, edible basement membrane solution was prepared with sodium alginate (2.5 %), acacia (1 %), glycerol (2 %) and water (94.5 %). Zinc gluconate and ε-polylysine were used as antibacterial agents and membrane modification agents to prepare edible antibacterial film agent. The effects of the addition of ε-polylysine (ε-PL) and zinc gluconate (ZG) on the color, microstructure, water vapor transmission coefficient, mechanical properties and antibacterial activity of the film were investigated, and the application assay of cold meat preservation was conducted. The application test of cold meat packaging was compared with packaging performance of zinc-loaded and ε-PL antibacterial film and base film. The results showed that after adding ε-PL and ZG, the color of the antibacterial film was yellowish; the thickness and the vapor permeability of water vapor decreased; while the tensile strength, elongation at break and antibacterial activity increased. The results of comprehensive analysis showed that the antibacterial effect of the antibacterial film with a final concentration of ε-PL of 2.6 mg/mL and a final concentration of ZG of 0.39 mmol/L on Escherichia coli O157:H7 and Staphylococcus aureus was significant, and had relatively good physical properties. The thickness, tensile strength, elongation at break and water vapor permeability were (0.0976 ± 0.0006) mm, (10.75 ± 0.18) MPa, (29.34 ± 1.03) % and (3633.75 ± 62.03) g/(m2?d). The application assay showed that the number of bacteria in the test group was much lower than that of the control group after being placed in a 37 ℃ incubator for 24 h, indicating that the zinc-loaded and ε-PL antibacterial film had a good bactericidal effect. The results in this paper can provide a theoretical basis for the practical application of zinc-loaded and ε-PL antibacterial film.

Key words: Zinc gluconate, ε-polylysine, edible antimicrobial film, antibacterial properties, physical properties

CLC Number:

TS201.6

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