Structural Elucidation of Flavonoids in the Supercritical CO2 Extract of Black Goji Fruit  by Using Ultra-high Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry

doi:10.7506/spkx1002-6630-20190505-033

FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (10): 206-212.doi: 10.7506/spkx1002-6630-20190505-033

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Structural Elucidation of Flavonoids in the Supercritical CO2 Extract of Black Goji Fruit by Using Ultra-high Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry

SHU Tong, DING Lina, WANG Qian, JIA Caihui, QIU Yiyi, RUAN Hui,,,

(1. Qinghai Institute for Food Control, Xining 810008, China; 2. College of Biosystems Engineering & Food Science, Zhejiang University, Hangzhou 310058, China; 3. Zhejiang Key Laboratory for Agro-food Processing, Hangzhou 310058, China; 4. Ningbo Research Institute, Zhejiang University, Ningbo 315100, China)

Online:2020-05-25 Published:2020-05-15

Abstract

Abstract: The flavonoid components of the supercritical CO2 extract of black Goji berries from Qaidam, Qinghai were analyzed by ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) employing PeakView workstation, and their structures were determined by analysis of the tandem mass spectra, ultraviolet (UV) spectroscopic analysis and comparison of the mass spectral data with the reference standards. As a result, a total of 13 flavonoids were identified, including 7 flavonol aglycones and flavonol glycosides, 3 dihydroflavonol aglycones, 2 flavonoid aglycones and 1 dihydrogen, namely rutin, quercetin-3-β-glucuronide, hyperoside, quercetin-3-O-galactopyranoside, dihydroquercetin, dihydroiso rhamnosin, dihydrokaempferol, quercetin, apigenin, hesperetin, alizarin, kaempferol, isorhamnetin, and kaempferol. It is noteworthy that hesperetin and alizarin were found in black Goji for the first time.

Key words: black Goji, flavonoid, supercritical CO2 extract, ultra-high performance liquid chromatography coupled with tandem mass spectrometry

CLC Number:

TS201.2

SHU Tong, DING Lina, WANG Qian, JIA Caihui, QIU Yiyi, RUAN Hui,,,. Structural Elucidation of Flavonoids in the Supercritical CO2 Extract of Black Goji Fruit by Using Ultra-high Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry[J]. FOOD SCIENCE, 2020, 41(10): 206-212.

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Structural Elucidation of Flavonoids in the Supercritical CO2 Extract of Black Goji Fruit by Using Ultra-high Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry

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