食品科学 ›› 2009, Vol. 30 ›› Issue (7): 194-196.doi: 10.7506/spkx1002-6630-200907044

• 生物工程 • 上一篇    下一篇

中性蛋白酶水解玉米蛋白粉制备可溶性肽最佳条件的研究

刘新华1,3,赵晨霞2,李治龙1,*,王鹏飞2,王晓娟3,胡彩香3   

  1. 1.新疆塔里木大学生命科学学院 2.北京农业职业学院园艺系 3.新疆农业大学食品科学学院
  • 收稿日期:2008-06-26 修回日期:2008-11-28 出版日期:2009-04-01 发布日期:2010-12-29
  • 通讯作者: 李治龙 E-mail:lizhilong405@126.com

Optimization of Production Conditions of Soluble Peptides from Corn Gluten Meal Hydrolyzed with Neutral Protease

LIU Xin-hua1,3 ZHAO Chen-xia2 LI Zhi-long1,* WANG Peng-fei2 WANG Xiao-juan3 HU Cai-xiang3   

  1. (1.College of Life Science, Tarim University, Alaer 843300, China 2.College of Horticulture, Beijing Vocational College of
    Agriculture, Beijing 102442, China 3. College of Food Science, Xinjiang Agricultural University, Urumqi 843001, China)
  • Received:2008-06-26 Revised:2008-11-28 Online:2009-04-01 Published:2010-12-29
  • Contact: LI Zhi-long1,* E-mail:lizhilong405@126.com

摘要:

通过对酶法水解玉米粉蛋白制备多肽的工艺进行了研究,实验以水解度(DH)和氮溶解指数(NSI)为指标确定了最佳酶解工艺条件。结果表明,在pH7.5、酶解温度50℃、酶与底物比2%、底物浓度5%的条件下酶解4h,可使水解度和氮溶解指数分别达到22.74%和23.86%。

关键词: 玉米蛋白粉, 酶解, 优化

Abstract:

The technology of producing polypeptide from corn gluten meal (CGM) via enzymolysis was studied. With degree of hydrolysis (DH) and nitrogen solubility index (NSI) as evaluation indexes, the optimum production conditions were determined as follows: substrate concentration 5%, concentration ratio of neutral protease to substrate 2%, hydrolysis temperature 50 ℃, pH 7.5, and hydrolysis time 4 h. Under these conditions, DH and NSI are 22.74% and 23.86%, respectively.

Key words: corn gluten meal, enzymolysis, optimization

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