食品科学 ›› 2010, Vol. 31 ›› Issue (6): 145-147.doi: 10.7506/spkx1002-6630-201006032

• 分析检测 • 上一篇    下一篇

发光细菌法检测液态奶中的三聚氰胺

张国辉1,2,赵 吉1,*,邵玉琴1,于景丽1,刘 芳1   

  1. 1.内蒙古大学环境与资源学院 2.呼和浩特市环境科学研究所
  • 收稿日期:2009-04-27 出版日期:2010-03-15 发布日期:2010-12-29
  • 通讯作者: 赵吉 E-mail:nmgzhaoji@yahoo.com
  • 基金资助:

    国家“973”计划前期研究专项(2009CB125909);内蒙古自治区自然科学基金项目(20080404Zd13)

Application of Luminescent Vibrio fischeri to Rapid Determination of Melamine in Liquid Milk

ZHANG Guo-hui1,2,ZHAO Ji1,*,SHAO Yu-qin1,YU Jing-li1,LIU Fang1   

  1. 1. College of Environment and Resources, Inner Mongolia University, Hohhot 010021, China;
    2. Hohhot Environmental Science Research Institute, Hohhot 010030, China
  • Received:2009-04-27 Online:2010-03-15 Published:2010-12-29
  • Contact: ZHAO Ji1 E-mail:nmgzhaoji@yahoo.com

摘要:

以发光的费氏弧菌为检测菌种,应用SKALAR TOXTRACER 毒素分析仪测定液态奶中三聚氰胺对费氏弧菌的毒性效应。结果表明:发光强度的抑制率与三聚氰胺溶液质量浓度呈正相关,相关系数在0.83~0.99 之间;发光强度的抑制率与液态奶中的三聚氰胺质量浓度呈负相关,相关系数在0.79~0.94 之间。发光菌对液态食品中三聚氰胺等毒性物质的检测具有快速、简便、灵敏和低廉的特点,发光菌的生物毒性检测法有望在食品安全方面得到广泛应用。

关键词: 费氏弧菌, 三聚氰胺, 液态奶

Abstract:

A method based on the toxicity effect of melamine on luminescent bacterium Vibrio fisheri measured using Skalar ToxTracer system was developed for determining melamine in liquid milk. Results showed that there was a positive correlation between inhibitory rate of melamine against luminescent intensity of Vibrio fisheri and melamine concentration in aqueous solution with a correlation coefficient ranging within 0.83-0.99, whereas inhibitory rate of melamine against luminescent intensity of Vibrio fisheri was negatively correlated with melamine concentration in liquid milk, and the correlation coefficient was between 0.79 and 0.94. This method, thanks to the characteristics of rapidity, simplicity, sensitivity and low cost, is expected to gain an extensive application to food safety detection.

Key words: Vibrio fischeri, melamine, milk

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