关键词: 多克隆抗体, 酶联免疫吸附反应(ELISA), 羊乳掺假, 牛乳

Abstract:

An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection and quantification of bovine milk adulteration in goat milk. The polyclonal antibodies were modified by mixed them with goat milk for the assay. The absorbance at 450 nm in indirect ELISA revealed a linear relationship with the concentration of adulterated bovine milk at the range of 4%－50%. Detection limit was 4% for mixed milk samples. The assay was characteristics of high reproducibility with intra- and inter-assay variation coefficients less than 5%. The recovery rate of spiked samples was in the range of 94%－105%. Therefore, the ELISA can successfully use to determine the adulteration of milk samples, which is suitable for developing a kit in routine inspection of milk.