蓝鲨软骨Ⅱ型胶原蛋白的物理化学特性

doi:10.7506/spkx1002-6630-201309006

食品科学 ›› 2013, Vol. 34 ›› Issue (9): 24-27.doi: 10.7506/spkx1002-6630-201309006

蓝鲨软骨Ⅱ型胶原蛋白的物理化学特性

宋瑞瑞1,2，包斌3，卜永士1，王永先1，陈丽娟1，吴文惠2,3,*

1.上海海洋大学食品学院，上海 201306；2.上海水产品加工与贮藏工程技术研究中心，上海 201306；
3.上海海洋大学海洋科学研究院，上海 201306

收稿日期:2012-05-29 修回日期:2013-04-07 出版日期:2013-05-15 发布日期:2013-05-07
通讯作者: 吴文惠 E-mail:whwu@shou.edu.cn
基金资助:
国家“863”计划项目(2011AA09070109)；上海市教委重点学科建设项目(J50704)

Physico-chemical Characteristics of Type Ⅱ Collagen Isolated from Prionace glauca Cartilage

SONG Rui-rui1,2，BAO Bin3，BU Yong-shi1，WANG Yong-xian1，CHEN Li-juan1，WU Wen-hui2,3,*

1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China；
2. Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center, Shanghai 201306, China；
3. Institute of Marine Science, Shanghai Ocean University, Shanghai 201306, China

Received:2012-05-29 Revised:2013-04-07 Online:2013-05-15 Published:2013-05-07
Contact: WU Wen-hui E-mail:whwu@shou.edu.cn

摘要/Abstract

摘要：

目的：对采用碱处理-胃酶水解方法分离的蓝鲨软骨Ⅱ型胶原蛋白的物理化学特性进行研究。方法：蓝鲨软骨经冷冻干燥粉碎，顺次用碱处理、胃蛋白酶限制性水解、盐析和透析获得Ⅱ型胶原蛋白。采用SDS-PAGE、氨基酸成分分析、紫外光谱扫描和差示扫描量热法对提取的Ⅱ型胶原蛋白进行鉴定。结果：蓝鲨Ⅱ型胶原蛋白由分子质量为130kD的α1链构成，甘氨酸是蓝鲨Ⅱ型胶原蛋白含量最高的氨基酸为356‰，富含丙氨酸、脯氨酸和羟脯氨酸，且肽链氨基酸残基具有Gly-X-Y的构成特点，蓝鲨Ⅱ型胶原蛋白在226nm波长处有紫外吸收峰，变性温度为41℃。结论：采用碱处理-限制性酶水解分离得到蓝鲨Ⅱ型胶原蛋白，具有Ⅱ型胶原蛋白的典型特征。

关键词: Ⅱ型胶原蛋白, 限制性酶解, SDS-PAGE, 氨基酸分析, 紫外光谱分析

Abstract:

Objective: To isolate type Ⅱ collagen from Prionace glauca cartilage using alkali treatment/pepsin hydrolysis
method, and to determine its biological activity. Methods: The freeze-dried Prionace glauca cartilage was crushed into 60－
200 mesh powder. Then, NaOH treatment and hydrolysis with pepsin were conducted. After salting and dialysis, the type Ⅱ
collagen was obtained. SDS-PAGE, amino acid composition analysis, UV spectroscopic scanning and differential scanning
calorimetry were applied to identify the type Ⅱ collagen. Results: Prionace glauca type Ⅱ collagen was composed of α1
chain and had the molecular weight of 130 kD. Glycine revealed the highest population in Prionace glauca type Ⅱ collagen
with the ratio of 356‰. Meanwhile, type Ⅱ collagen from Prionace glauca was rich in alanine, proline and hydroxyproline.
Moreover, type Ⅱ collagen from Prionace glauca had the characteristics of Gly-X-Y chain, UV absorption peak at
226 nm, thermal denaturation temperature of 41 ℃. Conclusion: Type Ⅱ collagen from Prionace glauca separated by alkali
treatment/enzyme hydrolysis has typical characteristics of type Ⅱ collagen.

Key words: typeⅡ collagen, restriction proteolysis, SDS-PAGE, amino acid analysis, UV spectroscopic analysis

中图分类号:

TS201.7

宋瑞瑞1,2，包斌3，卜永士1，王永先1，陈丽娟1，吴文惠2,3,*. 蓝鲨软骨Ⅱ型胶原蛋白的物理化学特性[J]. 食品科学, 2013, 34(9): 24-27.

SONG Rui-rui1,2，BAO Bin3，BU Yong-shi1，WANG Yong-xian1，CHEN Li-juan1，WU Wen-hui2,3,*. Physico-chemical Characteristics of Type Ⅱ Collagen Isolated from Prionace glauca Cartilage[J]. FOOD SCIENCE, 2013, 34(9): 24-27.

参考文献

[ ]Elsaid K.A., Chichester C.O., Review: Collagen markers in early arthritic diseases[J]. Clinica Chimica Acta, 2006, 365: 68 - 77.
[ ] David Eyre. Collagen of articular cartilage[J]. Arthritis Research 2002;4:5-30.
[ ] 李卫林, 曹健, 汤克勇, 等. 胶原蛋白结构和稳定性关系研究[J]. 中国皮革, 2005, 34(23): 14 -16.
[ ] Berg R A,Prockop D J,The thermal translation of a non-hydroxylated form of collagen.Evidence for a role for hydroxproline in stabilizing the triple-helix of collagen.Biochem.Res.commun[J].1973,52:115 - 120.
[ ] Heidemann E.,Roth W.,Sythesis and investigation of collagen model peptide[J]. Polym. Sci.,1982,43:143 - 203.
[ ] 姜旭淦, 陈盛霞, 丁建霞, 等. 猪软骨Ⅱ型胶原蛋白的提取纯化与鉴定[N]. 江苏大学学报(医学版). 2006, 16(5): 389 - 391.
[ ] 刘媛, 王健, 王永刚, 张志胜. 羊软骨Ⅱ型胶原蛋白的提纯工艺. 食品研究与开发[J]. 2009, 30(11): 109 -111.
[ ] 杜红丽, 马新. 鸡软骨Ⅱ型胶原蛋白的提纯与鉴定[J]. 中外医疗, 2010, 7: 52-53.
[ ] Miller, J. M. (1971). Isolation and characterization of a collagen fromchick cartilage containing three identical chains. Biochemistry[J]. 9:1652-1658.
[ ] Rigo, C., Hartmann, D. J., & Bairati, A. (2002). Electrophoretic and immunochemical study of collagens from Sepia officinalis cartilage.Biochemical et Biophysica Acta[J]. 1572, 77-84.
[ ] Takaoka, K., Koezuka, M., & Nakahara, H. (1991). Telopeptide-depleted bovine skin collagen as a carrier for bone morphogenetic protein.Journal of Orthop Research[J]. 9, 902-907.
[ ] Sivakumar, P., & Chandrakasan, G. (1998). Occurrence of a novel collagen with three distinct chains in the cranial cartilage of the squid Sepia of?cinalis: comparison with shark cartilage collagen. Biochimica et Biophysica Acta – General Subjects,1381(2), 161 - 169.
[ ] Phanat K., Soottawat B., Wonnop V., Fereidoon S. Isolation and characterization of collagen from the cartilages of brownbanded bamboo shark (Chiloscyllium punctatum) and blacktip shark (Carcharhinus limbatus). Food Science and Technology[J]. 2010,43: 792 - 800.
[ ] 肖旭, 石文达, 姚青, 等. 羊软骨Ⅱ型胶原蛋白的提取纯化与鉴定[N]. 宁夏医科大学学报, 2009, 31(6): 730 - 731.
[ ] 宁志刚, 王富友, 崔运利, 等. 可溶性猪软骨Ⅱ型胶原蛋白的提取与鉴定[J]. 重庆医学, 2011, 40(10): 954 - 955.
[ ] 陆雪芹, 许时婴, 曹慧. 鸡胸软骨粉中Ⅱ型胶原的提取及结构分析[J]. 食品科学, 2009, 30(4): 76 - 80.
[ ] 庞捷. 乌梢蛇Ⅱ型胶原蛋白提取和纯化及对AA大鼠干预作用的研究[D]. 广州: 南方医科大学, 2009.
[ ] 李斯明, 叶春婷, 邹海燕, 彭燕豪, 杨小红. 高纯度猪软骨Ⅱ型胶原的制备与检测[J].生物医学工程杂志,2001,18(4):592 - 594.
[ ] WANG Lin, AN Xinxin, YANG Fangmei, et al. Isolation and characterization of collagen from the skin of deep-sea red?sh (Sebastes mentella)[J].Food Chemistry,108(2):616 - 623.
[ ] Phanat Kittiphattanabawon, Soottawat Benjakul, Wonnop Visessanguan, Takashi Nagai, Munehiko Tanaka. Characterisation of acid-soluble collagen from skin and bone of bigeye snapper (Priacanthus tayenus)[J].Food Chemistry,89(3), 363-372.
[ ] Hui Cao, Shi-Ying Xu. Purification and characterization of type II collagen from chick sternal cartilage[J]. Food Chemistry 108 (2008) 439 - 445.
[ ] 蒋挺大.胶原蛋白[M].南京:化学化工出版社,2001:1 - 9.

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蓝鲨软骨Ⅱ型胶原蛋白的物理化学特性

Physico-chemical Characteristics of Type Ⅱ Collagen Isolated from Prionace glauca Cartilage

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